FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5809090
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG77001813.255398005539595Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3154915.430988330358112No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA335710.5779046287800671No Hit
GTTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280090.4821581349230258No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG245320.42230366546223247Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CTTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216030.3718826873055849No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204340.35175905348341996No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186440.3209452771432359No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTGAA173770.29913463210244634TruSeq Adapter, Index 1 (96% over 32bp)
ATTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171490.2952097488591156No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA165840.28548361275173906No Hit
TTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162320.27942414388484255No Hit
CGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA141330.24329111788593394No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTGAT132570.2282113033194528TruSeq Adapter, Index 1 (96% over 32bp)
CTCCGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA129790.22342570006661974No Hit
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT117380.2020626294307714Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113480.1953490133566531No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTGAC108290.18641473965801875TruSeq Adapter, Index 1 (96% over 32bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTGAG104780.18037248519131224TruSeq Adapter, Index 1 (96% over 32bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC97400.16766825785105757Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAAATCCAG94460.16260722419518375Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90140.15517060331308347No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85760.14763069602984288No Hit
CTCCATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84980.1462879728150192No Hit
AGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76220.13120815824853807No Hit
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA76140.13107044304701768No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTACAG76040.1308982990451172Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74060.12748984780748793No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCAGAACTCCAG70710.12172302374382218Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTGACTC67410.11604227168110669TruSeq Adapter, Index 7 (97% over 36bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA67130.11556026847578536No Hit
TTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66150.11387325725716077No Hit
CAAGCAGAAGACGGCATACGAGATAGGAAGAGCACACGTCTGAACTCCAG63940.1100688748151604Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GTTTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63040.10851957879805614No Hit
TCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62580.10772771638931398No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCTGATGC61400.10569641716688845TruSeq Adapter, Index 7 (97% over 36bp)
CCTGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58270.10030830990740375No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)