FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5809090
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG87611615.081811436903198Illumina Single End PCR Primer 1 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3831856.596299936823151No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG553580.9529547657206205No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349120.60098913943492No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA326890.5627215278124457No Hit
GTTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249710.4298607871456631No Hit
GATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203210.3498138262619447No Hit
CTTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193140.3324789252705673No Hit
TTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168260.28964949759773045No Hit
ATTTTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164720.28355559993045387No Hit
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT129030.22211740565217614Illumina Single End PCR Primer 1 (100% over 31bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA119280.20533336546688036No Hit
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG108480.1867418132616296Illumina Single End PCR Primer 1 (100% over 32bp)
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107950.18582945005155713No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGAAAGAGT100950.17377936991852425Illumina Single End PCR Primer 1 (96% over 26bp)
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC93970.16176371858587146No Hit
CTCCATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA93800.16147107378264067No Hit
TTTTTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85790.14768233923041302No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85340.14690769122186093No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84950.14623632961444907No Hit
CTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83670.14403288639012304No Hit
TTTTTCTAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82380.141812228765607No Hit
CTCCGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81710.14065886395287386No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGGGTAGGGAAAGAG80310.1382488479262673Illumina Single End PCR Primer 1 (96% over 30bp)
CGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78310.13480596788825788No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA75760.13041629583979591No Hit
GTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73840.12711113100330687No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTGTATGCGTGT72980.12563069258696283Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAGAGT66650.1147339772666631Illumina Single End PCR Primer 1 (96% over 26bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTGTGTAGGGAAAGAGT65860.1133740396516494No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGGAGGGAAAGAG64160.11044759161934141Illumina Single End PCR Primer 1 (96% over 30bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA62840.10817529079425521No Hit
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA61510.10588577556897896No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGGTCGTGTAGGGAAAGAGT60550.10423319315073444No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)