FastQCFastQC Report
Wed 8 May 2024
EGAF00007947450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503310
Total Bases75.9 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG26240.521348671792732Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12770.25372037114303314No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATCGTAA5170.10271999364208936TruSeq Adapter, Index 7 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)