FastQCFastQC Report
Wed 8 May 2024
EGAF00007947460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9005185
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1107861.230246796706564Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAA497670.5526482798521074TruSeq Adapter, Index 25 (97% over 38bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAAC490370.5445418389516706TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAAT447050.4964362198000374TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAAA434070.48202230159624704TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACATA426300.4733939391583849TruSeq Adapter, Index 25 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAT424460.4713506718629323TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACACT421790.4683857133418136TruSeq Adapter, Index 25 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAC377620.4193361935373899TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACACA360500.4003249239188312TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAAC355020.39423954088672253TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACATC341570.3793037011455067TruSeq Adapter, Index 25 (97% over 38bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT331840.3684988148494451Clontech SMART CDS Primer II A (100% over 26bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACATT306200.3400263292758561TruSeq Adapter, Index 25 (97% over 38bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACA280340.31130954000389777TruSeq Adapter, Index 25 (97% over 38bp)
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT260340.28910011287941334Clontech SMART CDS Primer II A (100% over 26bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACACC240490.2670572564583626TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAGC198010.21988443324595774TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAAG179760.19961833099486573TruSeq Adapter, Index 25 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAG179030.19880768690482206TruSeq Adapter, Index 25 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA174940.19426585905786498No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAGT164730.1829279465108157TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAGA151880.16865838958333448TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACATG150310.16691494955406247TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACACTC125570.13944188820107528TruSeq Adapter, Index 25 (97% over 38bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAA122800.13636588254433418TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACATCT117260.130213871230852TruSeq Adapter, Index 25 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACACG113850.1264271639061274TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAACT108860.12088591183856856TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACACTT107240.11908694824148533TruSeq Adapter, Index 25 (97% over 38bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA102950.11432302612328343No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAAAT98450.10932590502027444TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACACTA93350.10366250110353092TruSeq Adapter, Index 25 (97% over 38bp)
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG92590.1028185428728005No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAGG92530.10275191459142705TruSeq Adapter, Index 25 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACACAT90960.10100847456215503TruSeq Adapter, Index 25 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)