FastQCFastQC Report
Wed 8 May 2024
EGAF00007947461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9005185
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1099441.220896627887156Illumina Single End PCR Primer 1 (100% over 30bp)
CTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGAGAACGTGTA582760.6471382875532262Illumina Single End PCR Primer 1 (97% over 34bp)
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT296390.3291326052712965Clontech SMART CDS Primer II A (96% over 26bp)
GCTAATCATTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT248440.27588550374034515Clontech SMART CDS Primer II A (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG224600.24941186660795972No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA119350.1325347563653606No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT109290.12136341452174497No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA92050.10221888834043942No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)