FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132965
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG44990.2109270428722459No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37530.17595225425639896No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG35010.16413771440225228Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC24430.11453540025269987No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC22400.10501813203685947No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)