FastQCFastQC Report
Wed 8 May 2024
EGAF00007947469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132965
Total Bases322 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG56370.26428000459454326No Hit
GGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGGACTGGTGTAG49310.2311805397650688Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG38100.17862459065197975Illumina Single End PCR Primer 1 (100% over 30bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT36840.17271732072490642No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27130.1271938358107142No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA23140.10848748104164858No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)