FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10077283
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAAA791950.7858765105634128TruSeq Adapter, Index 12 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA745260.73954457764062No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCAA646210.641254195203211TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAAC614210.6094996042087932TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAAT575900.5714834048026636TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCTA540250.536106805772945TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTAA506710.5028240250869207TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCAT504150.5002836578073673TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCAC499390.4955601623969476TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGATA472120.4684992968838922TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGACA454910.4514212809147069TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCTC445690.44227198938444023TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTAC431000.42769464745606534TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTAT392160.3891525126365906TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCCA383510.3805688497584121TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTCA364410.3616153282586189TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTCT362430.35965051294083933TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCTT347310.34464646869597687TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGACC344440.34179847881616504TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCCC335000.3324308744728117TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAAG329270.3267448180228738TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGATT321310.31884586351301236TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTTA317260.3148269230902814TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGAA317240.3148070764709099TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGACT316060.3136361259279907TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTGC275840.2737245743718818TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGATC273370.27127351687950013TruSeq Adapter, Index 12 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG273160.2710651273760993Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAGC271580.2694972444457499TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGAC262800.26078457854165654TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGCA261920.25991132728931TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAGA260790.25878999329481966TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGCT259420.25743049986787114TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGATG254540.2525879247412224TruSeq Adapter, Index 12 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAA252630.2506925725912431TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCAG247000.24510574923816272TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCCT243210.24134481486726136TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTGA234550.232751228679397TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGTA232820.2310344961037613TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGACG230170.22840481903703608TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTGT229500.22773995728809043TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCGC223850.22213328731563856TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGAT220590.21889828835808223TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTTT219620.21793572731856392TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTCC218910.2172311723308753TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCTG218660.21698308958873141TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCGA217480.21581213904581226TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGCC210570.20895513205295516TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAGT201910.20036154586509083TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTAG201720.20017300298106147TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCGT200100.19856542681196904TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTTC191390.18992222407567594TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGTC176180.17482887004364173TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGAGG173460.1721297298091162TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGTG169410.16811078938638518TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTTG169080.16778332016675526TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGTT161140.15990421227626533TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGGC146830.1457039561159491TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTGG136480.13543333059119209TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGCGG133480.13245633768546541TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGGA122180.12124299774056162TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTCG111420.11056551651868862TruSeq Adapter, Index 12 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGTA108800.10796560938102066TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGCG105850.10503823302372277TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGGT105650.10483976683000765TruSeq Adapter, Index 12 (97% over 38bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTC105550.1047405337331501TruSeq Adapter, Index 12 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTGTTCGGAG103560.10276579510568473TruSeq Adapter, Index 12 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)