FastQCFastQC Report
Wed 8 May 2024
EGAF00007947471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10077283
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA612200.6075050189619563No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG290750.28852022911334335Illumina Single End PCR Primer 1 (100% over 30bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCAACTTCGTGT116180.11528901192910829Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)