FastQCFastQC Report
Wed 8 May 2024
EGAF00007947480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4988831
Total Bases753.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG421840.8455688316561535Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGT153960.308609371614312TruSeq Adapter, Index 9 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGC85310.17100198423237828TruSeq Adapter, Index 9 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTG69540.13939137244777383TruSeq Adapter, Index 9 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGA58730.11772296956942417TruSeq Adapter, Index 9 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGCA58660.11758265613727946TruSeq Adapter, Index 9 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGT55280.1108075218422913TruSeq Adapter, Index 9 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATGTGTGCT51690.10361144725086899TruSeq Adapter, Index 9 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)