FastQCFastQC Report
Fri 10 May 2024
EGAF00007947484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5823970
Total Bases879.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG98580.1692659817959227Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66980.11500746054667177No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)