FastQCFastQC Report
Wed 8 May 2024
EGAF00007947486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4829955
Total Bases729.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG714601.4795168899089122Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGTGT130200.2695677288918841TruSeq Adapter, Index 12 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75890.15712361709374104No Hit
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGTGTAA74480.15420433523707777TruSeq Adapter, Index 12 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGTGTAAA60150.12453532175765612TruSeq Adapter, Index 12 (97% over 38bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTGTGTA55890.11571536380773735TruSeq Adapter, Index 12 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)