FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10569324
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1350171.2774421524025568Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980270.9274670735801078No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCTC644530.6098119425613219TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTACT489570.4631989709086409TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTCT454220.42975312328394893TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTAC417090.39462315659923TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCTA408710.386694551136856TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGCT407510.3855591899727929TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCTT395900.3745745707104825TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTAAC385830.3650469982753864TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCAC383610.36294658012186964TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGCC369290.3493979368973834TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCCC359240.339889287148355TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTACC344680.32611357169105615TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTTC341130.32275479491403614TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGCA320050.3028102837986611TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTCC309840.29315025256109095TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCAA308060.29146613350106404TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCCT305370.2889210322249559TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTAA300650.2844552783129744TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTTT300160.28399167250431534TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCAT296410.28044366886661815TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTACA293400.27759580461342653TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTTA286190.27077417628601413TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTAT285820.27042410659376137TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTATC282580.267358631450791TruSeq Adapter, Index 7 (97% over 35bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT273520.25878665466211465Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCCA264300.2500632963848965TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTCA257870.2439796528141251TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTAAT251840.23827446296470806TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTAAA246380.233108569668221TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTGC243390.23027962810109712TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTATT236110.22339177037244765TruSeq Adapter, Index 7 (97% over 35bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG231560.21908685929204175No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGTC216550.2048853833982192TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTATA210590.1992464229500392TruSeq Adapter, Index 7 (97% over 35bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA189460.17925460511949487No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTCTG173660.16430568312599747TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGAC169510.16037922576694594TruSeq Adapter, Index 7 (97% over 35bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA156210.1477956395319133No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTGT149960.14188230013575134TruSeq Adapter, Index 7 (97% over 35bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTT143320.1355999683612689TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGAA142510.13483359957552632TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTGA139690.13216550083997802TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTTTG131170.12410443657513007TruSeq Adapter, Index 7 (97% over 35bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC130610.12357460136523395Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGTA127530.12066050771080533TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGAT124880.11815325180683268TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTGTT123150.11651643946197505TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTAGC115790.10955289098905474TruSeq Adapter, Index 7 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGTTATG115240.10903251712219249TruSeq Adapter, Index 7 (97% over 35bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTGT114910.10872029280207514TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)