FastQCFastQC Report
Wed 8 May 2024
EGAF00007947493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10569324
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1349121.2764487113840015Illumina Single End PCR Primer 1 (100% over 30bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA537230.5082917318080135No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGCGTGT304780.28836281298595823Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT278180.263195640515893Illumina Single End PCR Primer 1 (100% over 31bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG244910.2317177522422437No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA185170.1751956889579693No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA159950.15133418182657662No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)