FastQCFastQC Report
Wed 8 May 2024
EGAF00007947495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5169206
Total Bases780.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGGCTGATTGTCACTTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGA242240.4686212930960771Illumina Single End PCR Primer 1 (100% over 29bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG186280.36036482198620057Illumina Single End PCR Primer 1 (100% over 30bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG137950.26686883827032626No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT110500.2137659052473436No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79950.15466591967896037No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA68950.13338605580818408No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66720.12907204704165398No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA55540.10744396721662863No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG54640.1057028874453833No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)