FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5015116
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA302710.6035952109582311No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCAA266190.530775359931854TruSeq Adapter, Index 8 (97% over 38bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG244030.48658894430358135Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCTA215010.42872388196005834TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCAC204040.4068500110466039TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCTC173050.3450568242090512TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCAT171260.34148761464341004TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCCA170080.339134727890641TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGACT161310.32164759499082374TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGACA148450.2960051173292901TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCCT139190.2775409382355264TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCCC137470.27411130669759187TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCTT136760.27269558670228167TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGAAA130610.2604326599823414TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGACC123410.24607606284680156TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGAAC123160.2455775698907064TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTCT102350.20408301622534755TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGATC101430.20224856214691744TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTAC100690.20077302299687586TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGAAT98350.19610712892782542TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGATA97530.19447207203183337TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTAA92040.18352516671598423TruSeq Adapter, Index 8 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGAA84480.16845073972366742TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTCA76830.15319685526715635TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGGCT72470.1445031381128572TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGATT67770.1351314705382687TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTAT67450.13449339955446693TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGGCA67220.1340347860348594TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGGCC65940.13148250209965232TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCGA64830.12926919337458995TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGAGC62390.12440390212310143TruSeq Adapter, Index 8 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCA61410.12244980973520853TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCTG59600.11884072073307976TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTTA57750.11515187285797578TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGGAC57470.11459356074714921TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGTCC56290.11224067399438017TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGAGA53460.10659773373138327TruSeq Adapter, Index 8 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTGTAGCAG50650.10099467290487399TruSeq Adapter, Index 8 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)