FastQCFastQC Report
Fri 10 May 2024
EGAF00007947497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5015116
Total Bases757.2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG255450.5093601025380071Illumina Single End PCR Primer 1 (100% over 30bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170720.3404108698582446No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCTTGGAAGTGT79720.15895943383961605Illumina Single End Sequencing Primer (100% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76180.151900773581309No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)