FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7789895
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG658800.8457110140765697Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAAA366100.469967823699806TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCACT343990.44158489941135276TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAAT308170.3956022513782278TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCACA296710.3808908849220689TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAAC287320.3688368071713419TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTAA283150.3634837183299646TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAA277000.3555888750746961TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCATA271470.3484899347167067TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGCA252210.3237655963270365TruSeq Adapter, Index 1 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250390.3214292362092172No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCAA250040.3209799361865596TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTCT242990.31192975001588596TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGCT241410.30990148134217466TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTTA228830.2937523548135116TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGAA221210.28397045146308136TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCACC219070.281223302753118TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCATC219040.2811847913226045TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCTC215150.27619114249935334TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTAC211740.27181367656431826TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCATT200270.2570894729646549TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCTA193650.24859128396467473TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTCA189690.2435077751368921TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCAC185000.2374871548332808TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCCA184180.23643450906591168TruSeq Adapter, Index 1 (97% over 36bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGT183810.2359595347562451TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTAT183380.23540753758555152TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCAT180970.2323137860009666TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTGC179760.23076049163692192TruSeq Adapter, Index 1 (97% over 36bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA175700.22554861137409427No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTTT175030.22468852275929263TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAAG169210.21721730523967267TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCCT168300.21604912518076302TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTGA161590.20743540188924242TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTA158670.20368695598592793TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGTA157760.20251877592701828TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGAC157420.20208231304786523TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCATG151820.19489351268534427TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAGA150180.192788221150606TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGAT150020.19258282685453398TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGCC149490.1919024582487954TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTTC144790.18586900080167962TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCCC138410.17767890324580754TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGTC138360.17761471752828503TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAT137250.17618979459928535TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAC137100.17599723744671783TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTGT131190.16841048563555736TruSeq Adapter, Index 1 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCA128480.1649316197458374TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTCC127010.16304455965067563TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCTT125540.16115749955551392TruSeq Adapter, Index 1 (97% over 36bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTT124280.1595400194739467TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAGC120840.1551240421083981TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCAGT104900.13466163536222248TruSeq Adapter, Index 1 (97% over 36bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAC104490.1341353124785379TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCACG103910.1333907581552768TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCA100840.1294497550993948TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTAG99730.12802483217039512TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGTT99220.1273701378516655TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGA99200.1273444635646565TruSeq Adapter, Index 1 (97% over 36bp)
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG98880.12693367497251246No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTTG97790.12553442633052178TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGCG94700.12156774898763077TruSeq Adapter, Index 1 (97% over 36bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTC92120.11825576596346933TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGC91490.11744702592268574TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGGA90100.11566266297556001TruSeq Adapter, Index 1 (97% over 36bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCT87970.11292835140910115TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCGC87760.11265877139550662TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGTG86680.11127235989702043TruSeq Adapter, Index 1 (97% over 36bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTT83230.10684354538796736TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCCAG82370.10573955104658023TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGAG80790.10371128237286896TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCGGC80770.10368560808585996TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGTTCTCG78660.1009769708064101TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)