FastQCFastQC Report
Wed 8 May 2024
EGAF00007947499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7789895
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG691360.8875087533272272Illumina Single End PCR Primer 1 (100% over 30bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA166210.2133661621883222No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTAGCTCAGTGT158620.2036227702684054Illumina Single End Sequencing Primer (100% over 33bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA156270.2006060415448475No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG116120.14906491037427333No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG114290.14671571311294954No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTAGCTCAGTGTAGATC96060.12331360050424299Illumina Single End Sequencing Primer (100% over 33bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT90850.11662544873839763No Hit
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT78700.10102831938042811Illumina Single End PCR Primer 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)