FastQCFastQC Report
Wed 8 May 2024
EGAF00007947506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8728796
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1204211.3795831635886553Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGACGT142860.1636651836060781TruSeq Adapter, Index 7 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGA137860.15793701674320262TruSeq Adapter, Index 7 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGACGTGAA105630.1210132531451073TruSeq Adapter, Index 7 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGACGTGA96070.11006099810328938TruSeq Adapter, Index 7 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)