FastQCFastQC Report
Wed 8 May 2024
EGAF00007947507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8728796
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1244031.4252022844845957Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98100.11238663384961682No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCACCAAGTGT94160.10787283836167096Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)