FastQCFastQC Report
Wed 8 May 2024
EGAF00007947518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3109422
Total Bases469.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA155730.5008326306303873No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG118600.3814213702739609Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG64540.20756269171569508No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACTCTCAA33720.10844459195310253TruSeq Adapter, Index 18 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACTCTC33020.10619336970022081TruSeq Adapter, Index 18 (97% over 37bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA32720.1052285601632715No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)