FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313855
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1054364.5567246002882635Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTT622462.6901426407445586TruSeq Adapter, Index 19 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCT610852.639966635765854TruSeq Adapter, Index 19 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTA390931.689518141802317TruSeq Adapter, Index 19 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTT356191.5393790881451086TruSeq Adapter, Index 19 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTC289121.2495164995213617TruSeq Adapter, Index 19 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTG230980.9982475133489351TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAC206770.8936169293235747TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTA179850.7772742890111957TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTT176900.7645250026470976TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCT172960.747497142215048TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAT163510.7066562079300561TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAA158790.6862573497474993TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTC143160.618707740977719TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCA138000.596407294320517TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTGC128900.5570789872312656TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCC125320.5416069719148348TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTGA108700.4697787890771029TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTG102830.44440987010854177TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAG96800.4183494644219279TruSeq Adapter, Index 19 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGA95380.4122125197992096TruSeq Adapter, Index 19 (100% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTGT93950.40603235725661285TruSeq Adapter, Index 19 (97% over 37bp)
GACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAG66100.285670450395552TruSeq Adapter, Index 19 (97% over 37bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGC63280.2734829969898719TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTACT61900.2675189240466667TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTGG53880.23285815230427143TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTCT51100.2208435705781045TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCTT50270.21725648322820576TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTACC49490.21388548547769848TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCG47190.20394536390568987TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTAC46510.20100654535396556TruSeq Adapter, Index 19 (97% over 37bp)
GGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTA45640.19724658632455364TruSeq Adapter, Index 19 (97% over 37bp)
GGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTT45260.19560430536917825TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAAC44890.19400524233368122TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTTC41620.1798729825334777TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTTT40300.1741682171095423TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCTA40030.17300133327282824TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTAT39210.1694574638428078TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCCC38810.16772874704767585TruSeq Adapter, Index 19 (97% over 37bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG38090.16461705681643837Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTGCT37640.16267225042191494TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTATT36540.15791827923530213TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCAT36240.15662174163895318TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTATC35980.15549807572211743TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCCT35740.15446084564503826TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCAC35350.1527753467697846TruSeq Adapter, Index 19 (97% over 37bp)
GGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTC34960.151089847894531TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTACA34850.1506144507758697TruSeq Adapter, Index 19 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC34460.14892895190061609Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCT33250.14369958359534196TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTTA32540.14063111128398278TruSeq Adapter, Index 19 (97% over 37bp)
CAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC32450.1402421500050781Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAAT32330.13972353496653853TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCTC32090.13868630488945935TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTAA31250.13505599961968232TruSeq Adapter, Index 19 (97% over 37bp)
GAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT30940.13371624410345506TruSeq Adapter, Index 1 (97% over 35bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTGCC30630.1323764885872278TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTGAC30510.13185787354868822TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTATA29000.12533196764706517TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCCA27960.12083730397972214TruSeq Adapter, Index 19 (97% over 37bp)
CATACGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGC27710.11975685598276468TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTCC27470.11871962590568554TruSeq Adapter, Index 19 (97% over 37bp)
GGCATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTA27130.11725021662982338TruSeq Adapter, Index 19 (97% over 37bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG27030.11681803743104041TruSeq Adapter, Index 15 (97% over 36bp)
CAAGCAGAAGACGGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT26860.11608333279310933Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTCA26790.11578080735396125TruSeq Adapter, Index 19 (97% over 37bp)
GGATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTG26410.11413852639858592TruSeq Adapter, Index 19 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCCG25470.11007604193002588Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTTGC25440.10994638817039097TruSeq Adapter, Index 19 (97% over 37bp)
CAAGCAGAAGAGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT25230.10903881185294671Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAGC24860.10743974881744967TruSeq Adapter, Index 19 (97% over 37bp)
GGCATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTT24760.10700756961866668TruSeq Adapter, Index 19 (97% over 37bp)
CAAGAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT24660.10657539041988369Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTAAA24530.10601355746146582TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCAA24130.1042848406663339TruSeq Adapter, Index 19 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAGCTTCTG23140.10000626659838235TruSeq Adapter, Index 19 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)