FastQCFastQC Report
Wed 8 May 2024
EGAF00007947521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313855
Total Bases349.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC64

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG984284.253853417781149Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG223730.9669145214371687No Hit
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGTAGAT205540.8883011251785441Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAA185790.8029457334189049Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAG143740.6212143803306603Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGT131070.5664572758448563Illumina Single End PCR Primer 1 (97% over 35bp)
CGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGTAG124750.5391435504817718Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTG115240.49804330867751Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGT104010.449509584654181Illumina Single End PCR Primer 1 (97% over 35bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGTAGA99810.4313580583052957Illumina Single End PCR Primer 1 (97% over 35bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGTAGATC84580.3655371663306473Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGAT48000.20744601541583205Illumina Single End PCR Primer 1 (97% over 35bp)
CGTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGTA40990.1771502535811449Illumina Single End PCR Primer 1 (97% over 35bp)
CTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGTAGA36940.15964699603043406Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATC36560.15800471507505873Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGTGTAGATC34560.14936113109939905Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG28460.12299819997363708Illumina Single End PCR Primer 1 (100% over 32bp)
CGTATCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGATCAAGT25810.111545451205888Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)