FastQCFastQC Report
Wed 8 May 2024
EGAF00007947523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4759539
Total Bases718.6 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1216822.5565921405413423Illumina Single End PCR Primer 1 (100% over 30bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGTAGGGTGT386550.8121584884586511Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT308990.6492015298120258Illumina Single End PCR Primer 1 (100% over 31bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100820.21182723789005617No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGTAGGGTGTAGATC93000.19539707522094052Illumina Single End Sequencing Primer (100% over 33bp)
CGTATGCCGTCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTC92260.1938423027944513Illumina Single End Sequencing Primer (100% over 33bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGTAGGGTGTAGATC77900.16367131354528242Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG68670.144278679090559Illumina Single End PCR Primer 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)