FastQCFastQC Report
Fri 10 May 2024
EGAF00007947524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5885612
Total Bases888.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG145410.2470601188117735Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCGCAGTGCC126750.21535568433665012TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCGCAGTGCT91080.15475026216475024TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCGCAGTCTC86530.1470195452911269TruSeq Adapter, Index 3 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72540.12324971472805206No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCGCAGTGCA69910.11878119046923243TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCGCAGTACT59610.10128088633773345TruSeq Adapter, Index 3 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCGCAGTACC59530.10114496164544996TruSeq Adapter, Index 3 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)