FastQCFastQC Report
Wed 8 May 2024
EGAF00007947529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5937694
Total Bases896.5 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG310210.5224418772675049Illumina Single End PCR Primer 1 (100% over 30bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA205180.34555502523370185No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT59430.10008936129076373No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)