FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4578262
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG337250.7366332464153427Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATGATACGGCGACCACCGAGATCTACACTGGAGTTGACACTCTTTCCCT145700.3182430363312541Illumina Single End PCR Primer 1 (96% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA114500.250094905009805No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCCAT71320.15577963864890212TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCCATGTGC62450.1364054743918107TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCCATGCTC55440.12109398719426717TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCCATGTAC50980.11135229919126517TruSeq Adapter, Index 9 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCCATGACT49520.10816331612301786TruSeq Adapter, Index 9 (97% over 37bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCCA45900.10025638550174718TruSeq Adapter, Index 9 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)