FastQCFastQC Report
Thu 9 May 2024
EGAF00007947535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4578262
Total Bases691.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG430610.9405534239849096No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG365260.7978136681561693Illumina Single End PCR Primer 1 (100% over 30bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAACTCCAGTGTAGATCT138410.30231996333980016Illumina Single End Sequencing Primer (100% over 32bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62680.13690784843680856No Hit
GAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAACTCCAGTGTAG54320.1186476440186254Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)