FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6781457
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG586030.8641653261238699Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA323180.47656425455473655No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCAA142540.2101908188756487TruSeq Adapter, Index 19 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCTA132900.19597558459782316TruSeq Adapter, Index 19 (97% over 38bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGG119190.17575869020477458TruSeq Adapter, Index 19 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCAT109540.16152870983329984TruSeq Adapter, Index 19 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCT108850.16051122937150525TruSeq Adapter, Index 19 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCA106840.15754726454801674TruSeq Adapter, Index 19 (97% over 38bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAG91940.1355755850107138TruSeq Adapter, Index 19 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCTC89990.13270009674912045TruSeq Adapter, Index 19 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCTCT88200.13006054598591424TruSeq Adapter, Index 19 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCAC88090.12989833895577307TruSeq Adapter, Index 19 (97% over 38bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGC87010.12830576084165984TruSeq Adapter, Index 19 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCTT81910.12078525308056956TruSeq Adapter, Index 19 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCTCA80380.11852910075224247TruSeq Adapter, Index 19 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCCA75740.11168691329901524TruSeq Adapter, Index 19 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCTAC71700.10572949146473981TruSeq Adapter, Index 19 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCACT69340.10224941336352941TruSeq Adapter, Index 19 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)