FastQCFastQC Report
Thu 9 May 2024
EGAF00007947542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3912978
Total Bases590.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG222180.5678028345674317Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTA112730.2880925985272598TruSeq Adapter, Index 5 (97% over 38bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTT85670.2189381080087851TruSeq Adapter, Index 5 (97% over 38bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCT84760.2166125135382821TruSeq Adapter, Index 5 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTT72250.18464197856466355TruSeq Adapter, Index 5 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTAA71440.18257194392608392TruSeq Adapter, Index 5 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61250.15653039705308847No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTAT50720.12961994675155342TruSeq Adapter, Index 5 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTTA50400.1288021552893985TruSeq Adapter, Index 5 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTG50090.12800991981043594TruSeq Adapter, Index 5 (97% over 38bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTAC49330.12606766508781803TruSeq Adapter, Index 5 (97% over 38bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGTCTTC47580.12159536802915835TruSeq Adapter, Index 5 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)