FastQCFastQC Report
Wed 8 May 2024
EGAF00007947544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2740099
Total Bases413.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG220140.803401628919247Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAC108860.3972849156180123TruSeq Adapter, Index 4 (97% over 39bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100080.3652422777425195No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACG96320.35152014580495083TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACA90290.32951364166039254TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACC69700.2543703712895045TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACT65480.2389694678914886TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACGC52880.192985727887934TruSeq Adapter, Index 4 (97% over 39bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA51580.18824137376058311No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACCC50000.18247515874426434TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACAC44600.1627678415998838TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAGC43510.15878988313925885TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACGT43460.1586074079805146TruSeq Adapter, Index 4 (97% over 39bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGA41000.1496296301702968TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACGA39730.14499476113819246TruSeq Adapter, Index 4 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAG39700.1448852760429459TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACCT39070.14258608904276815TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACTC38700.1412357728680606TruSeq Adapter, Index 4 (97% over 39bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAC38300.1397759715981065TruSeq Adapter, Index 4 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACGG35930.13112664907362837TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAGT35720.13036025340690247TruSeq Adapter, Index 4 (97% over 39bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAG35580.1298493229624185TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGATA32370.11813441777103674TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACTT32050.11696657675507344TruSeq Adapter, Index 4 (97% over 39bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAT31090.11346305370718357TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAGG30720.11211273753247601TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGATC29960.1093391151195632TruSeq Adapter, Index 4 (97% over 39bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGAGA28690.10470424608745889TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACAT28390.10360939513499329TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACTA28000.10218608889678803TruSeq Adapter, Index 4 (97% over 39bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTCCAGACCA27500.10036133730934539TruSeq Adapter, Index 4 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)