FastQCFastQC Report
Fri 10 May 2024
EGAF00007947551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5249386
Total Bases792.6 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTGAAGTGTA242130.46125394474706183Illumina Single End PCR Primer 1 (97% over 35bp)
TTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTGAAGTGTA176850.3368965437100644Illumina Single End PCR Primer 1 (97% over 35bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80890.1540942121611937No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70020.13338702850200004No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG64230.12235716710487664Illumina Single End PCR Primer 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)