FastQCFastQC Report
Wed 8 May 2024
EGAF00007947554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5230997
Total Bases789.8 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA899711.7199589294354403No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA344190.6579816428875795No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG239840.45849768218180975Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA236480.45207443246478635No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG207980.3975915107578919No Hit
CTTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC183390.3505832635728906No Hit
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC139870.26738688628573104No Hit
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC121610.23247958276405054No Hit
CGCCCTTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC114450.2187919434861079No Hit
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA82330.1573887348817061No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGGTGCA69350.13257510948677662TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGGTGCCC64990.12424017830635345TruSeq Adapter, Index 1 (97% over 36bp)
GGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG63600.12158294107222772No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGGTGCAC63090.12060798352589383TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGGTGCTC57710.11032313725280286TruSeq Adapter, Index 1 (97% over 36bp)
CGGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG54950.1050468964138194No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGGTGCTA54520.10422487338455748TruSeq Adapter, Index 1 (97% over 36bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACTGGTGCCA54170.10355578487236754TruSeq Adapter, Index 1 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)