FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8940092
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG2291512.563183913543619Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAC732060.8188506337518674TruSeq Adapter, Index 25 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTG685410.7666699626804736TruSeq Adapter, Index 25 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGT601830.6731809918734617TruSeq Adapter, Index 25 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTA530590.5934950110133095TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAA498180.5572425876601718TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCT467360.5227686694946764TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCC440260.49245578233423104TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGC439570.4916839781961975TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCA437530.4894021224837507TruSeq Adapter, Index 25 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTC417360.4668408334052938TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTA413110.46208696733769633TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTC397580.4447157814483341TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAT394240.44097980199756337TruSeq Adapter, Index 25 (97% over 37bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA393800.44048763703997673No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA376500.4211366057530504No Hit
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTT347100.38825103813249345TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTT340070.3803875843783263TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTACT329850.36895593468165655TruSeq Adapter, Index 25 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTG325180.3637322747909082TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCTC271890.3041243870868443TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTACC270160.30218928395815164TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCCT266710.2983302632679843TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGA265730.29723407768063237TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCCC245800.2749412422154045TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGCC234760.2625923760068688TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTCT214080.23946062300030024TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGCT207700.23232423111529504TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTG201550.225445107276301TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTACA200740.22453907633165296TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAAC191410.21410294211737418TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCTT185050.20698892136680475TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAG180570.20197778725319607TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCAC178630.19980778721292802TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCCA176530.19745881809717394TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCTA168340.18829783854573307TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTCC164360.18384598279301823TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTAC164310.18379005495692885TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCAT162860.18216814771033676TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTCA160480.17950598271248214TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCAA160440.17946124044361064TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGCA159640.17856639506618052TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTTC155220.17362237435587913TruSeq Adapter, Index 25 (97% over 37bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTT147150.1645956216110528TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTATC146020.16333165251543272TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGT137910.15426015750173488TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTCG136600.15279484819619304TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTAA135460.15151969353335515TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAAT135180.1512064976512546TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGAC128170.14336541503152317TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTGC123540.13818649740964634TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTTA123080.13767196131762402TruSeq Adapter, Index 25 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTG120570.13486438394593703TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTTT119510.13367871382084212TruSeq Adapter, Index 25 (97% over 37bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA118870.132962837518898No Hit
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTT118600.13266082720401537TruSeq Adapter, Index 6 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTATT118280.1323028890530433TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAAA117280.13118433233125565TruSeq Adapter, Index 25 (97% over 37bp)
CAAGCAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGT109400.12237010536356896TruSeq Adapter, Index 25 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTA108510.12137458988117795TruSeq Adapter, Index 6 (96% over 33bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTTAT107980.1207817548186305TruSeq Adapter, Index 25 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTC107840.12062515687758023TruSeq Adapter, Index 6 (96% over 33bp)
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC105520.11803010528303289No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTATA105480.11798536301416138TruSeq Adapter, Index 25 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTGG94480.10568123907449722TruSeq Adapter, Index 25 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTGTTGTAGC89630.10025623897382711TruSeq Adapter, Index 25 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)