FastQCFastQC Report
Wed 8 May 2024
EGAF00007947559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8940092
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2267012.5357792738598213Illumina Single End PCR Primer 1 (100% over 30bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTATGCGGTGTGTAGATC540000.6040206297653313Illumina Single End Sequencing Primer (100% over 33bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTATGCGGTGTGTAGATC453310.5070529475535599Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG375990.4205661418249387No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA306750.34311727440836176No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA209300.23411392187015523No Hit
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC114510.12808593021190387No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTATGCGGTGTGT111620.12485330128593755Illumina Single End Sequencing Primer (100% over 33bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA105830.11837685786678706No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG102870.1150659299702956No Hit
CGTATGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTATGCGGT101170.11316438354325661Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)