FastQCFastQC Report
Wed 8 May 2024
EGAF00007947560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253988
Total Bases38.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG12790.5035670976581571Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4540.17874860229617148No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA3230.12717136242657134No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTCTTCTCT3080.12126557160180797TruSeq Adapter, Index 16 (97% over 38bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGTCTTCTGC2800.11024142872891633TruSeq Adapter, Index 16 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)