FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9850696
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG5385755.467380172933973Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAA761060.7725951546976986TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCTA533090.5411698828184324TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCAA510350.5180852195621507TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCA509050.5167655158579657TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCAT502980.5106035147161175TruSeq Adapter, Index 8 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTA501180.5087762326641692TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCTT491440.49888860644973715TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTCT437440.4440701448912849TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTACT429980.4364970759426542TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTA421540.4279291534324072TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAT406520.41268149986559327TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCCT406050.41220437621869566TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCCA403920.41004209245722334TruSeq Adapter, Index 8 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTC400400.40646874088896867TruSeq Adapter, Index 8 (97% over 37bp)
GGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCT394820.4008041665279286TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAC373350.37900875227496617TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAAC363510.3690196103909815TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTACA356400.36180184628578527TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCAC354420.359791836028642TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTTT349810.35511196366226305TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCT336370.3414682576743816TruSeq Adapter, Index 8 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA333220.33827051408347186No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTATT331030.33604732092026796TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTACC323250.3281494018290687TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTAC319520.32436286735475345TruSeq Adapter, Index 8 (97% over 37bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGC314770.31954087305100065TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAAA312980.3177237425660075TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCTG308490.3131656890030918TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAAT303730.3083335431323837TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTTA302370.3069529300264672TruSeq Adapter, Index 8 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTT297640.302151238856625TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTAT290120.2945172605062627TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTCA288260.2926290690525827TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTAA288020.29238543144565626TruSeq Adapter, Index 8 (97% over 37bp)
AGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTT283270.28756343714190347TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTTC279340.2835738713284828TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCTC271730.27584852887552314TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTATA268600.27267108841852394TruSeq Adapter, Index 8 (97% over 37bp)
AGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG266310.27034637958576735Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCCC264700.268711977305969TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGCT263190.26717909069572343TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTATC259270.263199676449258TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTT257640.26154497103554913TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCGA255340.2592101106358373TruSeq Adapter, Index 8 (97% over 37bp)
AAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCT252590.25641842972313833TruSeq Adapter, Index 8 (97% over 36bp)
ACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAG251520.2553322120589246TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCGT234210.237759849659354TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGA233000.2365315100577665TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTCC225920.22934420065343605TruSeq Adapter, Index 8 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT213850.21709125933842646Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTC194660.19761040235126534TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGC194550.19749873511475738TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCAG191430.19433144622471346TruSeq Adapter, Index 8 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC188320.19117430890162482Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGAC188240.19109309636598268TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCC186970.1898038473626635TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGAA184180.18697156018214348TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCCG178510.18121562171850597TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTGC178330.18103289351331114TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAG175920.1785863658770913TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCG174870.1775204513467881TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCGC174560.17720575277117473TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGCA174340.17698241829815883TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAGC170070.17264769920825898TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTGA168590.1711452672988792TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGAT161800.16425235333625157TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGCC155200.15755231914577406TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAGA144780.1469743863783838TruSeq Adapter, Index 8 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTG140550.14268027355630505TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAAG133260.135279781245914TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTCGG130880.13286370831056TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTTG130260.1322343111593333TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTAG129550.13151354990550923TruSeq Adapter, Index 8 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTG124730.12662049463306957TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGTT123540.12541245816539257TruSeq Adapter, Index 8 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGAGATCGGAAGAGCACACGTCTGAACTCC122230.12408260289425235Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTGT119880.12169698465976414TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTACG116590.11835711913148067TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGTA116130.11789014705153829TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTATG113500.11522028494230256TruSeq Adapter, Index 8 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT110540.11221542112354295Illumina PCR Primer Index 2 (96% over 31bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTGC109220.11087541428544745TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTAGT102160.10370840801502756TruSeq Adapter, Index 8 (97% over 37bp)
CAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC100400.10192173223090024Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTTAGCTTCG99810.10132278978053937TruSeq Adapter, Index 8 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)