FastQCFastQC Report
Wed 8 May 2024
EGAF00007947565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9850696
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG5404435.486343300006416Illumina Single End PCR Primer 1 (100% over 30bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTCAGTGGTGT787170.7991008960179057Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG388390.39427670897569067No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA235410.2389780376939863No Hit
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT212770.2159948901072574Illumina Single End PCR Primer 1 (100% over 31bp)
CGTATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTCAGTGGTG202430.20549816987550928Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTG188650.19150931061114868Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG182070.1848295795545817Illumina Single End PCR Primer 1 (100% over 32bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTCAGTGGTGTAGATC181310.1840580604659813Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTCA169760.17233300063264567Illumina Single End PCR Primer 1 (97% over 35bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTCAGTGGTGTAGATC162670.16513553966135996Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTCAGTGGT156510.1588821744169143Illumina Single End PCR Primer 1 (97% over 35bp)
CGTATGCCGTCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTC141810.14395937099266892Illumina Single End PCR Primer 1 (97% over 35bp)
CTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAG121390.12322987127000974Illumina Single End PCR Primer 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)