FastQCFastQC Report
Wed 8 May 2024
EGAF00007947566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6140419
Total Bases927.2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA278530.4536009676212649No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTTCTCAAA90660.14764464770237992TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTTCTCTAA86590.1410164355233739TruSeq Adapter, Index 8 (97% over 37bp)
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT85470.13919245575912653No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTTCTCTCT83600.1361470609741778TruSeq Adapter, Index 8 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG78960.12859057337943877Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG74330.12105037131830906No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTTCTCATA74210.12085494491499685TruSeq Adapter, Index 8 (97% over 37bp)
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC70380.11461758554261525No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTTCTCAAC67210.10945507138845084TruSeq Adapter, Index 8 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTTCTCAAT65710.10701224134704813TruSeq Adapter, Index 8 (97% over 37bp)
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA62810.10228943660033622No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)