FastQCFastQC Report
Wed 8 May 2024
EGAF00007947568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471678
Total Bases71.2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22220.4710840870254708No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG8470.1795716569354517Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA6130.12996154156013212No Hit
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGGCATAA5990.12699341499921557TruSeq Adapter, Index 3 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)