FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3549680
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA316650.8920522413287958No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG92980.2619391043699714Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG40290.11350318901985532No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)