FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13164524
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1790901.360398598536491Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGAGTGTAATGATACGGCGACCACCGAGATCTACACCGACCATTACACTC251870.1913248059709565Illumina Single End PCR Primer 1 (96% over 29bp)
AAGAGTGTAATGATACGGCGACCACCGAGATCTACACCGACCATTACACT222160.16875657638665856Illumina Single End PCR Primer 1 (96% over 29bp)
AGTGTAATGATACGGCGACCACCGAGATCTACACCGACCATTACACTAGA192360.14611998124656841Illumina Single End PCR Primer 1 (96% over 29bp)
GTGTAATGATACGGCGACCACCGAGATCTACACCGACCATTACACAGATC189620.14403862988133867Illumina Single End PCR Primer 1 (96% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA141930.10781248148432863No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)