FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6207012
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG1423672.2936478937047324Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA227250.36611819020166225No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)