FastQCFastQC Report
Wed 8 May 2024
EGAF00007947575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6207012
Total Bases937.2 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1518842.4469744862745553Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132260.21308159223793993No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA124990.20136903231377673No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)