FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5515461
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG55738310.105827962522081Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA364750.6613227797277508No Hit
CAAGCAGAAGACGGCATACGAGATTAGATCGGAAGAGCACACGTCTGAAC230860.4185688195420111RNA PCR Primer, Index 29 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATTTAGATCGGAAGAGCACACGTCTGAA227420.4123318068970119Illumina PCR Primer Index 11 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAGATCGGAAGAGCACACGTCTGAACT164060.2974547367844682Illumina PCR Primer Index 2 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATATAGATCGGAAGAGCACACGTCTGAA147160.2668135990808384Illumina PCR Primer Index 6 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTAAGATCGGAAGAGCACACGTCTGAA120560.21858553618636775RNA PCR Primer, Index 32 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAAGATCGGAAGAGCACACGTCTGAAC112340.2036819769009336Illumina PCR Primer Index 10 (96% over 29bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAA98470.17853448696310245TruSeq Adapter, Index 4 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTA88860.16111073942867152TruSeq Adapter, Index 4 (96% over 33bp)
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCT87270.1582279341654306TruSeq Adapter, Index 4 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG74800.13561876332730846Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATTCAGATCGGAAGAGCACACGTCTGAA72180.13086848044071023RNA PCR Primer, Index 40 (96% over 29bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTT71350.12936361983159703TruSeq Adapter, Index 4 (96% over 33bp)
CAAGCAGAAGACGGCATACGAGATGTAGATCGGAAGAGCACACGTCTGAA69650.12628137521052185RNA PCR Primer, Index 39 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAAAGATCGGAAGAGCACACGTCTGAA67270.12196623274101656Illumina PCR Primer Index 2 (96% over 27bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTGCT63020.11426062118832858TruSeq Adapter, Index 4 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTAT62500.11331781695129382TruSeq Adapter, Index 4 (97% over 37bp)
GCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTA59850.10851314151255897TruSeq Adapter, Index 4 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTG59260.10744342132053876TruSeq Adapter, Index 4 (96% over 33bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCCTCTTTA57460.10417986819234148TruSeq Adapter, Index 4 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC56520.10247556822539404Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)