FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5515461
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG58030410.521405191696578Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG398250.7220611296136442No Hit
TAATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG329790.597937325637875Illumina PCR Primer Index 4 (96% over 26bp)
TATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA312070.5658094581758443Illumina Single End PCR Primer 1 (100% over 25bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA219060.3971744157016068No Hit
TTATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG177170.3212242820681716Illumina PCR Primer Index 2 (96% over 26bp)
AATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA172940.31355493221690806Illumina Single End PCR Primer 1 (100% over 25bp)
AAATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG165480.30002931758560164Illumina PCR Primer Index 4 (96% over 26bp)
GAATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG130460.23653507839145266Illumina PCR Primer Index 2 (96% over 26bp)
ATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA127300.23080572956639525Illumina Single End PCR Primer 1 (100% over 26bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTATGCCTGTGTAGATC115540.20948384912883983Illumina Single End PCR Primer 1 (96% over 33bp)
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTATGCCTGTGT109800.199076740820033Illumina Single End PCR Primer 1 (96% over 33bp)
TTCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAA108350.196447767466763Illumina Single End PCR Primer 1 (100% over 26bp)
ATATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG107560.1950154302604986Illumina PCR Primer Index 2 (96% over 26bp)
TCATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG77210.13998829834895035Illumina PCR Primer Index 3 (96% over 26bp)
GTATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG75710.1372686707421193Illumina PCR Primer Index 2 (100% over 26bp)
TGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTATGCCTGTGTAGATC68350.12392436461793493Illumina Single End PCR Primer 1 (96% over 33bp)
CATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA66670.12087838169828416Illumina Single End PCR Primer 1 (100% over 25bp)
TGATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG65930.11953669874558083Illumina Paired End PCR Primer 2 (96% over 26bp)
ACATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGG65070.11797744558433103Illumina PCR Primer Index 3 (96% over 26bp)
GATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGA58260.10563033624931806Illumina Single End Adapter 1 (96% over 25bp)
CGTATGCCGTCTTCTGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG55900.1013514554812372Illumina Single End PCR Primer 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)