FastQCFastQC Report
Thu 9 May 2024
EGAF00007947582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3194491
Total Bases482.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG2979609.327307542891809Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA834232.6114645494383923No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA97530.30530685483227216No Hit
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA86940.27215603362163177No Hit
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC63920.2000944751448666No Hit
GGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG52430.1641263036896958No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA50160.15702032029515814No Hit
CGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCA48360.15138561980609744TruSeq Adapter, Index 13 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACA47960.1501334641418617TruSeq Adapter, Index 13 (97% over 35bp)
ACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC47850.1497891213341969No Hit
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC47140.14756654503017852No Hit
AAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGT46250.14478049867725407Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTCT45350.1419631484327237TruSeq Adapter, Index 13 (97% over 37bp)
AGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC36820.11526092889289719Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTAC36680.11482267441041469TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTCA36030.11278792145603166TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTAA35600.11144185411697827TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTTA35430.11090968795967808TruSeq Adapter, Index 13 (97% over 37bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACACCAG35090.10984535564507773Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTTT34940.10937579727098934TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACCTC34180.1069967015089415TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACACT33560.10505586022937613TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACTAT33090.10358457732389918TruSeq Adapter, Index 13 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACATC32790.1026454605757224TruSeq Adapter, Index 13 (97% over 37bp)
CAAGCAGAAGACGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCACT32730.10245763722608703TruSeq Adapter, Index 13 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)