FastQCFastQC Report
Wed 15 Mar 2023
EGAF00007947586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35752841
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3657671.023043175785667No Hit
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCACACGTCTGAACTCCAG2195090.6139623981210331Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1301140.3639263240647086No Hit
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1203300.3365606666054874No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1130390.3161678815957591No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCTCT840250.23501628863563598TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCCCT816730.22843779044020587TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCACT800900.22401017026870673TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCCTC799600.22364656280042194TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCCAA748420.20933161647210077TruSeq Adapter, Index 6 (97% over 37bp)
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC743410.20793032922894156No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCCAC738370.20652065104420653TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCCCA707210.19780525972747173TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCAAC682510.19089671783006TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCACC674470.1886479454877446TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCCAT669030.18712638808199886TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCCCC659320.18441051999196373TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCCTT633790.17726982871095473TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCACA627480.1755049339995107TruSeq Adapter, Index 6 (97% over 37bp)
GGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG616760.17250657087642352No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCCTA609420.17045358717087686TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCTAC596680.16689023398168554TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATC580250.1622947949786704TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCAAA567010.15859159276321566TruSeq Adapter, Index 6 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCAA560130.15666727016183135TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCTAA546830.15294728606322502TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCTCA532810.1490259193667994TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATT518290.1449647036441104TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATA508870.14232994798930804TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCTTC500440.13997209340650718TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCTTT491120.1373653075569575TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCTAT486110.13596402031379828TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCAAT472570.13217690868258553TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCTTA467390.13072807277049675TruSeq Adapter, Index 6 (97% over 37bp)
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCTCC460370.12876459244175867TruSeq Adapter, Index 6 (97% over 37bp)
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC444780.12440409980286603No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCTGC398490.11145687695140087TruSeq Adapter, Index 6 (97% over 37bp)
ACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC383720.10732573671558016No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCGCC372740.104254652098836TruSeq Adapter, Index 6 (97% over 37bp)
CGGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG369060.10322536326553741No Hit
ATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCGCT367080.10267156112153436TruSeq Adapter, Index 6 (97% over 37bp)
CATACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCTA365220.10215132274383454TruSeq Adapter, Index 6 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)