FastQCFastQC Report
Sun 5 May 2024
EGAF00007947587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007947587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35752841
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3784771.058592798261822No Hit
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2342120.6550864027840473Illumina Single End PCR Primer 1 (100% over 30bp)
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1296270.3625641945489031No Hit
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA1075000.3006754064663001No Hit
GCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC1003080.28055952252857336No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG981560.27454042043819676No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA968030.2707561057875093No Hit
CCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC851980.23829714679177524No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC847070.2369238293538687No Hit
CGTATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTTCGAGGTGT806340.2255317276744525Illumina Single End PCR Primer 1 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA685090.19161833880557913No Hit
GGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG571210.1597663245838282No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA467580.13078121540047685No Hit
CGGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG360150.10073325361752371No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)